Authors: Mayra A. Cañizares-Martínez, Mercedes A. Quintanilla-Mena, Flor Árcega-Cabrera, Victor Ceja-Moreno, Marcela Del Río-García, Sandy G. Reyes-Solian, Isajav Rivas-Reyes, Rafael F. Rivera-Bustamante & Carlos A. Puch-Hau

Abstract: The present study evaluates the endocrine effect in flatfish through vitellogenin (vtg) gene expression and its association with pollutants data obtained from fish muscle and sediment from two regions in the Gulf of Mexico (GoM): Perdido Fold Belt (northwestern) and the Yucatan Peninsula (southeast). The results revealed induction of vtg in male flatfish in both geographical regions with different levels and patterns of distribution per oceanographic campaign (OC). In the Perdido Fold Belt, vtg was observed in male fish during four OC (carried out in 2016 and 2017), positively associated with Pb,
V, Cd and bile metabolites (hydroxynaphthalene and hydroxyphenanthrene). In the Yucatan Peninsula, the induction of
vtg in males was also detected in three OC (carried out in 2016 and 2018) mainly associated with Ni, Pb, Al, Cd, V and
polycyclic aromatic hydrocarbons. Ultimately, estrogenic alterations could affect reproductive capacity of male flatfish in
the GoM.

Keywords: Environmental Monitoring, Endocrine Disruptors, Gene Expression, Perdido Fold Belt, Yucatan Peninsula